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binding buffer (annexin v-fitc/pi double staining cell apoptosis detection kit)  (Keygen Biotech)

 
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    Keygen Biotech binding buffer (annexin v-fitc/pi double staining cell apoptosis detection kit)
    Effect of taurine on the <t>apoptosis</t> of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.
    Binding Buffer (Annexin V Fitc/Pi Double Staining Cell Apoptosis Detection Kit), supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/binding+buffer+cell+apoptosis+kit/binding+buffer/pmc05840730-54-19-28
    Average 90 stars, based on 1 article reviews
    binding buffer (annexin v-fitc/pi double staining cell apoptosis detection kit) - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells"

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.8036

    Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.
    Figure Legend Snippet: Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Techniques Used: Control

    Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.
    Figure Legend Snippet: Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Techniques Used: Staining, Control

    Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.
    Figure Legend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.

    Techniques Used: Expressing, Standard Deviation, Control

    Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.
    Figure Legend Snippet: Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Techniques Used: Expressing, Derivative Assay, Inhibition, Control

    Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.
    Figure Legend Snippet: Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Techniques Used: Expressing, Derivative Assay, Control

    Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.
    Figure Legend Snippet: Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.

    Techniques Used: Expressing, Derivative Assay, Control

    Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.
    Figure Legend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.

    Techniques Used: Expressing, Derivative Assay, Control, Standard Deviation

    Related Articles

    other:

    Article Title: Assessing Radiosensitivity: Effects of Acute Ionizing Radiation on Inflammation and Apoptosis in Macrophage Cell Line (RAW 264.7)
    Article Snippet: 24 hours after the treatment, the cells were collected in 500 μl Binding Buffer (Annexin V-FITC/PI apoptosis detection kit, Jiangsu Keygen Biotechnology).

    Article Title: USP18 contributes to the proliferation and migration of ovarian cancer cells by regulating the AKT/mTOR signaling pathway.
    Article Snippet: A2780 and SKOV3 with the indicated transfections and treatment were harvested by trypsin treatment and then resuspended in binding buffer from Annexin VFITC/PI apoptosis kit (Nanjing KeyGen Biotech Co., Ltd., Nanjing, China).

    Article Title: Knockdown of PHLDA2 promotes apoptosis and autophagy of glioma cells through the AKT/mTOR pathway.
    Article Snippet: Pleckstrin homology like domain family A member 2 (PHLDA2) is an imprinted gene expressed in placenta and has been shown to be associated with tumor progression.. However, the effect of PHLDA2 on glioma cell growth has not been reported yet.. Data based on TCGA database showed that PHLDA2 was up-regulated in glioma tissues.

    Article Title: New Aspects of Radiotherapy in Rheumatoid Arthritis Biomarker: Radio Sensitivity Response to Acute Ionizing Radiation Induces Cell Characteristic Reprogramming and Enhanced Cytokine Release of In Vitro Activated Macrophage Cell Line (RAW 264.7)
    Article Snippet: 24 h after treatment, cells collected in 500 µl Binding Buffer (Annexin V-FITC/PI apoptosis detection kit, Jiangsu Keygen Biotechnology).

    Article Title: Synergistic Effects of Taurine and Cisplatin on Lung Cancer Cells (A549)
    Article Snippet: Lung cancer is one of the most common types of cancer that causes death.. In this study, the effects of cisplatin, taurine, and a combination of these two compounds on the A549 cell line were examined.. A549 cells were treated with different concentrations of taurine, cisplatin, and the combination of the two compounds.

    Binding Assay:

    Article Title: Prevention of acute graft-vs.-host disease by targeting glycolysis and mTOR pathways in activated T cells.
    Article Snippet: .. Cells were centrifuge at 400 x g for 5 min, and subsequently resuspended in Annexin V binding buffer (Cell Apoptosis Analysis Kit, KGA108, Nanjing KeyGen Biotech Co., Ltd). ..

    Article Title: Prevention of acute graft-vs.-host disease by targeting glycolysis and mTOR pathways in activated T cells
    Article Snippet: .. Cells were centrifuge at 400 x g for 5 min, and subsequently resuspended in Annexin V binding buffer (Cell Apoptosis Analysis Kit, KGA108, Nanjing KeyGen Biotech Co., Ltd). ..

    Analysis:

    Article Title: Prevention of acute graft-vs.-host disease by targeting glycolysis and mTOR pathways in activated T cells
    Article Snippet: .. Cells were centrifuge at 400 x g for 5 min, and subsequently resuspended in Annexin V binding buffer (Cell Apoptosis Analysis Kit, KGA108, Nanjing KeyGen Biotech Co., Ltd). ..



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    Image Search Results


    Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Control

    Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Staining, Control

    Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Standard Deviation, Control

    Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Inhibition, Control

    Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control

    Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control

    Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control, Standard Deviation